精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當前位置：首頁 >產(chǎn)品中心>細胞庫>人正常細胞>CRL-2888WPE-int 人正常前列腺上皮細胞

WPE-int 人正常前列腺上皮細胞

簡要描述：CRL-2888 WPE-int 人正常前列腺上皮細胞，原代細胞|細胞系|細胞株|菌種原代細胞、細胞系。細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件！

  • 產(chǎn)品型號：CRL-2888
  • 廠商性質(zhì)：生產(chǎn)廠家
  • 更新時間：2025-04-27
  • 訪  問  量：1541

產(chǎn)品分類

Product Category

詳細介紹

CRL-2888 WPE-int 人正常前列腺上皮細胞

ATCC® Number:CRL-2888™    Price:$329.00
Designations:WPE-int

Depositors:MM Webber, EJ Tokar

Biosafety Level:2 [cells contain human HPV-18 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial
WPE-int 人正常前列腺上皮細胞

Source:Organ: prostate, normal, peripheral zone
Cell Type: epithelial

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2888 WPE-int 人正常前列腺上皮細胞

Isolation:Isolation date: 2004 [PubMed: 16351690] [92794]

Receptors:androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)

Tumorigenic:NO

Antigen Expression:kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18



Age:54

Gender:male

Ethnicity:Caucasian

Comments:WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.

  1. The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:

  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.




CRL-2888

ATCC® Number: CRL-2888™ Price: $329.00
Designations: WPE-int
Depositors: MM Webber, EJ Tokar
Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial


Source: Organ: prostate, normal, peripheral zone
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794]
Receptors: androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)
Tumorigenic: NO
Antigen Expression: kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 14,18
Age: 54
Gender: male
Ethnicity: Caucasian
Comments: WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).
Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
•0.05 mg/ml BPE - provided with the K-SFM kit
•5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品：

  • 您的單位：

  • 您的姓名：

  • 聯(lián)系電話：

  • 常用郵箱：

  • 省份：

  • 詳細地址：

  • 補充說明：

  • 驗證碼：

    請輸入計算結(jié)果（填寫阿拉伯數(shù)字），如：三加四=7

聯(lián)系方式

郵箱：xiangfbio@163.com

地址：上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

（周一至周日9:00- 19:00）

在線咨詢

  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號：滬ICP備10013034號-2     sitemap.xml
技術(shù)支持：化工儀器網(wǎng)    管理登陸
男女男精品视频| 男女啪啪猛烈免费网站| 精品人妻系列无码专区| 国产成人亚洲精品无码h在线| 久久精品无码专区免费青青| 少妇暴力深喉囗交3p| 黑人女性猛交xxxxxⅹxx| 香蕉夜夜草草久久亚洲香蕉| 丰满人妻少妇久久久久久| 亚洲婷婷五月激情综合APP| 亚洲精品国产福利一二区| 亚洲欧美中文字幕日韩一区二区| 欧美xxxx做受视频| 最近更新中文字幕免费版| 色播在线| 国产精品久久久久久久美男 | 国产精品久久久久久精品三级| 天一影院| 国产精品国产三级国产| 欧美一级a一级a爰片免费免免| 丝袜一区二区三区| 香蕉超碰| 属虎人永远最旺的颜色| 国产视频久久久久| 尖峰时刻2| 久久无码高潮喷水| 亚洲国产成人久久精品软件| 亚洲av无码之日韩精品| 一区二区三区无码被窝影院| 成人无码一区二区三区| 亚洲综合另类小说色区色噜噜| 国产凹凸在线一区二区| 在线观看免费人成视频| 最近中文字幕在线mv视频7| 日本精品ΑV中文字幕| 中文无码伦AV中文字幕| 无码一区二区三区老色鬼| 潮喷失禁大喷水aⅴ无码| 国产亚洲日韩AV在线播放不卡| 国产v亚洲v天堂a_亚洲| 成人欧美一区二区三区视频|