精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>EA.hy926人臍靜脈細(xì)胞融合細(xì)胞

人臍靜脈細(xì)胞融合細(xì)胞

簡要描述:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):EA.hy926
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-25
  • 訪  問  量:4561

產(chǎn)品分類

Product Category

詳細(xì)介紹


ATCC® Number: CRL-2922™ Price:

Designations:

EA.hy926

Depositors: CS Edgell

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Source: Tissue: somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression: Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Comments: The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.

Atmosphere: air, 95; carbon dioxide (CO2), 5

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

CRL-2922 EA.hy926

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

6.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

7.Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.

Interval: Twice a week

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: Complete Growth medium, 95; DMSO, 5

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

CRL-2922 EA.hy926

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC 30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402

References: 92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

CRL-2922 EA.hy926

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270






























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久大香蕉视频| 滦平县| 热久久这里只有精品| 国色天香和国色天香哪个好| 久久性视频| 精品国产一区二区av麻豆| 国产精品久久久久久久久齐齐| 亚洲国产日韩A在线播放| 国产成人综合亚洲看片| 天堂网站| 久久av一区二区三区| 四虎影视在线播放| 少妇性xxxxxxxxx色孕交| 麻豆极品少妇av0077| 男人的天堂色偷偷| 懂色av一区二区三区在线播放| 粉嫩av国产一区二区三区| 大香蕉av在线| 国产精品久久久久久久久久软件| 无码国产午夜福利| 亚洲精品人成网线在播放va| 久久99久久99精品免视看动漫| 99久热re在线精品99re8热视频| 成人AV鲁丝片一区二区免费| 国内精品乱码卡一卡2卡麻豆| 无码一区二区三区久久精品| 亚洲精品无码成人片久久不卡| 99久久er这里只有精品18| 色99久久久久高潮综合影院| 郎溪县| 无线乱码一二三区免费看| 久久午夜无码免费| 午夜理论在线观看无码| 国产裸体歌舞一区二区| 国产成人一区二区三区在线观看| 中文字幕乱码亚洲无线码| 国产成人综合精品无码| 国产精品综合AV一区二区国产馆| 又嫩又硬又黄又爽的视频| 亚洲AV成人无码网站在线| 成人欧美一区二区三区黑人|