精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-119 NCI-H69 H69人肺小細(xì)胞肺癌細(xì)胞

HTB-119 NCI-H69 H69人肺小細(xì)胞肺癌細(xì)胞

簡要描述:HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞
HTB-119 NCI-H69 H69人肺小細(xì)胞肺癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問  量:3019

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞

ATCC® N
AF Gazdar
Bios
umber:
HTB-119™Price:
Designations:NCI-H69 [H69]

Depositors:


1
Shipped:frozen
Medium & Serum:See Propagation
afety Level:



Growth Properties:suspension, multicell aggregates

Organism:Homo sapiens

Morphology:floating aggregates


Source:Organ: lung
Disease: carcinoma; small cell lung cancer


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:insulin-like growth factor II (IGF II)

Tumorigenic:Yes  HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞

Oncogene:myc +; myb +; fes +; fms +; raf +; ras +

DNA Profile (STR):CSF1PO: 10, 12

D13S317: 12

D16S539: 11

D5S818: 11, 13

D7S820: 9

THO1: 8, 9

TPOX: 10

vWA: 16, 17

Amelogenin: XY



Cytogenetic Analysis:modal number = 76 to 78; range = 40 to 87

This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others.



Isoenzymes:AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 2

PGM3, 1



Age:55 years adult  HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞

Gender:male

Ethnicity:Caucasian

Comments:This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.

The cells grow in aggregates, thus cell counts are not accurate.

The cells stain positively for cytokeratins.

The line can be adapted to grow in shaker flask or spinner flask systems.

The N-myc gene is amplified, and there is expression of the mRNA and protein.

C-myc mRNA, but not protein, is expressed at a low level.

There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended  HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞
Medium Renewal: 2 times weekly


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derivative:ATCC CRL-11351

purified DNA:ATCC HTB-119D



References:

1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

22446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

22465: Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086

22869: Rygaard K, et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int. J. Cancer 54: 144-152, 1993. PubMed: 8386707

23036: Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

23037: . . Cancer Res. 40: 4556-4563, 1980.

23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

23057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

23065: Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

24389: . . Lung Cancer 4: 155-161, 1988.

32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久亚洲精品国产精品婷婷| 国内裸体无遮挡免费视频| 精品少妇一区二区三区免费观刮毛| 日日干| 人人妻人人妻人人片AV| 吃奶揉捏奶头高潮视频| 又粗又黄又爽视频免费看| 七台河市| 欧美v片| 美女扣逼视频| 少妇无码AV无码专区在线观看| 天天躁狠狠躁狠狠躁性色av| 亚洲不卡av一区二区无码不卡| 国产我和子的乱视频| 欧洲另类二三四区| 中文av人妻av无码中文| 国产精品午夜午码免费看| 18禁亚洲AV无码成人网站国产| 无码专区—va亚洲v天堂麻豆| 亚洲AV永久综合在线观看尤物| 玩弄人妻少妇精品视频| 亚洲av中文无码乱人伦在线咪咕| 色欲AⅤ亚洲情无码AV蜜桃| 久久精品国产99国产精品| 欧洲人妻丰满AV无码久久不卡| 国产成人久久精品一区二区三区| xxx| 四虎国产精品永久在线国在线| 精品丰满熟女一区二区三区| 动漫3d国漫| 国产av色图| 日韩人妻久久| 国产精品国产三级国产普通话| 高清无码色大片中文| 97亚洲熟妇自偷自拍另类图片| 久久精品午夜福利| 国产精品亚洲成在人线| 99精品国产综合久久久久五月天 | 开原市| 免费人成视频在线播放| 色综合av|